Substitution matrix — where it appears
Named by 6 essays across 2 fields — each of them below, with the objects they name alongside it.
A cost that is not one
The same eighty-one cells, filled by the same recurrence, return 6, 10, 10 and 15 — in edits, in cost, in bits and in bits again. Only the first is a count of anything, two of them are equal by arithmetic coincidence, and the alignment each one chooses is different.
The zero that moves the answer out of the corner
One extra term in the recurrence — a floor at zero — and the answer stops being in the last cell. It becomes a maximum over all 1,040 of them, the traceback's starting point is a search, and the whole mode is meaningless unless a randomly matched pair of characters scores negative on average. That last condition is on the scoring scheme, not on the sequences.
The matrix a corpus wrote
A substitution matrix is not a property of an alphabet. Fit one to four hundred pairs of sequences that rarely change and the dearest substitution costs five; fit the same model to four hundred pairs that often change and it costs two. Two hundred test pairs aligned under each matrix give different alignments in 115 cases — and a matrix fitted to eight pairs of the first kind moves 79 of them, from sampling alone.
The ties a rounded matrix makes
Measure how far each optimal alignment is from a tie — the smallest change to any one cost that makes another alignment win — and it predicts which alignments a refitted substitution matrix will move. A resample of the same corpus moves 30 of the 63 test alignments that sit on a tie and 3 of the other 137. A matrix fitted to a different divergence moves alignments far from a tie as well, and the prediction weakens to a chance of 0.62. And a third of the alignments were on a tie only because the matrix was rounded to whole bits — fitted without rounding, 15 of 200 are, and every prediction improves.
The lattice that decides the ties
Rounding a fitted substitution matrix to whole bits puts 63 of 200 alignments on a tie where the exact fit puts 15. Rounding to half bits — a finer grain, and the obvious repair — puts 79. What tracks the ties is not how fine the lattice is but how many of the six fitted costs it keeps apart: whole and half bits both leave three, an eighth of a bit leaves all six, and matches the exact fit exactly.
A distance that is not a distance
Under unit cost the edit distance obeys the triangle inequality and this site asserts that it does. Under a stated substitution matrix it need not, and on 42,840 enumerated triples it fails 2,832 times — taking with it every structure that prunes by distance, at a measured 13.22% of the bounds they rely on.
Named alongside it
The objects these essays reach for when they reach for this one.
AlignmentCost modelHonest limitEdit distanceParameter choiceCorpusEstimatorFittingMeasurementDynamic programmingLog oddsOptimality